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β‐Aminobutyric Acid Primed Expression of WRKY and Defence Genes in B rassica carinata Against A lternaria Blight
Author(s) -
Chavan Vinodkumar,
Kamble Avinash
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12132
Subject(s) - wrky protein domain , biology , gene expression , gene , priming (agriculture) , microbiology and biotechnology , genetics , botany , transcriptome , germination
Foliar spray with BABA led to a significant reduction of lesion development in B rassica carinata caused by A lternaria b rassicae . To get better insight into molecular mechanisms underlying priming of defence responses by BABA , expression pattern of B c WRKY genes and marker genes for the SA and JA pathway namely PR ‐1 and PDF 1.2 was examined. Q‐ RT ‐ PCR analysis revealed priming of B c WRKY 70, B c WRKY 11 and B c WRKY 53 gene expression in BABA ‐pretreated B rassica plants challenged with pathogen. However, the expression of B c WRKY 72 and B c WRKY 18 remained unchanged. Furthermore, B c WRKY 7 gene was found to be upregulated in water‐treated plants in response to pathogen indicating its role in susceptibility. In addition, BABA application potentiated expression of defence genes PR ‐1, PDF 1.2 and PAL in response to the pathogen. In conclusion, BABA ‐primed expression of B c WRKY 70, B c WRKY 11 and B c WRKY 53 genes is strongly correlated with enhanced expression of PR ‐1, PDF 1.2 and PAL hence suggesting their role in BABA ‐induced resistance.