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Applicability of AFLP Fingerprinting Markers to Molecular Discrimination of the Three Main Fungal Species Associated with Grapevine Decline in Spain
Author(s) -
Martín Laura,
Sáenz de Miera Luis E.,
Martín Maria Teresa
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12118
Subject(s) - amplified fragment length polymorphism , biology , population , botany , genetic diversity , demography , sociology
Diplodia seriata , Phaeomoniella chlamydospora and Phaeoacremonium aleophilum are the three main species associated with grapevine decline in Spain. AFLP markers were developed to discriminate Spanish populations of these species. The markers were used to genotype isolates of D. seriata , P. chlamydospora and P. aleophilum . AFLP markers were valuable in performing population genetic studies as genetic variability (K x ) ranged from 0.07 in the P. chlamydospora population to 0.28 in the D. seriata population. Species‐specific markers obtained using only two AFLP combinations clearly discriminate D. seriata , P. chlamydospora and P. aleophilum and are a useful tool in simultaneous identification tests.