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Race Differentiation in F usarium oxysporum f.sp. chrysanthemi
Author(s) -
Troisi Marco,
Bertetti Domenico,
Gullino Maria Lodovica,
Garibaldi Angelo
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12116
Subject(s) - biology , fusarium oxysporum , phylogenetic tree , ribosomal dna , botany , intergenic region , cultivar , pathogenicity , gerbera , chrysanthemum morifolium , canker , genetics , microbiology and biotechnology , gene , genome
The pathogenicity of different isolates of F usarium oxysporum obtained from plants of Gerbera ( G erbera jamesonii ), Chrysanthemum ( C hrysanthemum morifolium ), P aris daisy ( A rgyranthemum frutescens ) and African daisy ( O steospermum sp.), all in the family Asteraceae, was tested on different cultivars of these hosts, to assess their pathogenicity. The reactions were compared with those of isolates of F . oxysporum f. sp. chrysanthemi and of f.sp. tracheiphilum obtained from the A merican T ype C ulture C ollection. We found that isolates of F . oxysporum f. sp. chrysanthemi can be distinguished as three physiological races on the basis of their pathogenicity to the panel of differential cultivars. Sequencing of the intergenic spacer ( IGS ) region of ribosomal DNA (r DNA ) and phylogenetic analysis showed that the F usarium races fell into three phylogenetic groups, which coincided with those observed in pathogenicity tests. Analysis of the IGS sequences revealed a high degree of similarity among strains from I taly and S pain from different host species, suggesting that recent outbreaks in these ornamentals were probably caused by introduction of infected nursery material from a common origin.