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Occurrence and Phylogenetic Analysis of Potyviruses, Carlaviruses and Allexiviruses in Garlic in S udan
Author(s) -
Salih Mohammed Heyam,
Zicca Stefania,
Manglli Ariana,
Elaiderous Mohamed Moawia,
El Siddig Marmar A. R.,
El Hussein Adil Ali,
Tomassoli Laura
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12115
Subject(s) - biology , allium sativum , virus , phylogenetic tree , virology , potyvirus , primer (cosmetics) , plant virus , botany , genetics , gene , chemistry , organic chemistry
Allium sativum L. samples of three cultivars grown in Sudan were shown to contain several of the common garlic viruses of the genera P otyvirus , C arlavirus and A llexivirus . In particular, O nion yellow dwarf virus ( OYDV ), L eek yellow stripe virus ( LYSV ), G arlic common latent virus (Gar CLV ), Garlic virus A ( GVA ), Garlic virus B ( GVB ), G arlic virus C ( GVC ) and Garlic virus X ( GVX ) were detected by reverse transcription polymerase chain reaction ( RT ‐ PCR ) assay using degenerate genus‐specific and virus‐specific primer sets. Multiple viral infections with members of all three genera were found in all but one sample. Further, molecular and phylogenetic analysis of nucleotide and deduced amino acid sequences revealed that Sudanese isolates of LYSV , Gar CLV and of A llexivirus genus were significantly divergent in partial coat protein genomic region from isolates of the same species retrieved from G en B ank. This is the first report and study on viruses infecting garlic in Sudan.