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First Report of a 16SrI‐C Phytoplasma Infecting Celery ( Apium graveolens ) with Stunting, Bushy Top and Phyllody in the Czech Republic
Author(s) -
Fránová Jana,
Špak Josef
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12110
Subject(s) - phyllody , phytoplasma , apium graveolens , biology , aster yellows , sieve tube element , restriction fragment length polymorphism , ribosomal rna , polymerase chain reaction , ribosomal dna , botany , 16s ribosomal rna , phloem , genetics , gene , phylogenetics
Apium graveolens L. plants showing stunting, purplish/whitening of new leaves, flower abnormalities and bushy tops were observed in S outh B ohemia ( C zech Republic) during 2011 and 2012. Transmission electron microscopy observations showed phytoplasmas in phloem sieve tube elements of symptomatic but not healthy plants. Polymerase chain reactions with universal and group‐specific phytoplasma primers followed by restriction fragment length polymorphism analyses and sequencing of 16S r DNA enabled classification of the detected phytoplasmas into the aster yellows group, ribosomal subgroup 16 S rI‐ C . Identical analyses of the ribosomal protein genes rpl22 and rps3 were used for further classification and revealed affiliation of the phytoplasmas with the rp IC subgroups. This is the first report of naturally occurring clover phyllody phytoplasma in A. graveolens in both the Czech Republic and worldwide.