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Mixed Infection of Two Groups (16 S r I & V ) of Phytoplasmas in a Single Jujube Tree in C hina
Author(s) -
Sun XianChao,
Mou HaiQing,
Li TingTing,
Tian Qian,
Zhao WenJun
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12101
Subject(s) - biology , orchard , phytoplasma , restriction fragment length polymorphism , microbiology and biotechnology , polymerase chain reaction , horticulture , gene , genetics
Symptoms similar to Jujube witches' broom disease were observed on jujube ( Z izyphus jujuba ) plants in an orchard in X uchang C ity, H enan P rovince, C hina. Phytoplasmas were detected in one sample from symptomatic plants by nested PCR assay employing 16 S rRNA gene primers P 1/ P 7 followed by R 16 F 2n/ R 16 R 2. Virtual RFLP analysis of the resulting products ( F 2n R 2 region) shown that total of two different groups (16 S r I and 16 S r V ) phytoplasmas associated with the infected jujube. This is the first report of phytoplasmas mixed infection of jujube in C hina.