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Detection of Aster Yellows Phytoplasma (16 S rI) Associated with Prickly Ash ( Z anthoxylum schinifolium S . et Z .) witches' Broom Disease in K orea
Author(s) -
Han SangSub,
Lee KuiJae,
KamalaKannan Seralathan
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12094
Subject(s) - phytoplasma , aster yellows , biology , phyllody , 16s ribosomal rna , broom , botany , phylogenetic tree , polymerase chain reaction , bacteria , genetics , restriction fragment length polymorphism , gene , ecology
Prickly ash trees with shortened internodes, proliferation of shoots, phyllody and witches' brooms were observed for the first time in K orea. A phytoplasma was detected in infected trees by polymerase chain reaction amplification of 16S rDNA , 16 S –23 S intergenic spacer region and the fragment of rp operon sequences. The 16S rDNA sequences exhibited maximum (99.6%) similarity with Iranian lettuce phytoplasma, and the sequences of rp operon exhibited maximum (100%) similarity with golden rain phytoplasma. Based on the sequence analysis and phylogenetic studies, it was confirmed that phytoplasma infecting prickly ash trees in K orea belongs to the aster yellows group (subgroup 16 S r I ‐ B ).