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Detection and Molecular Variability of Apple Stem Grooving Virus in Shaanxi, China
Author(s) -
Liu Ping,
Zhang Lei,
Zhang Huawei,
Jiao Honghong,
Wu Yunfeng
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12083
Subject(s) - biology , coat protein , phylogenetic tree , serology , host (biology) , plant virus , china , horticulture , botany , virus , virology , gene , genetics , geography , rna , antibody , archaeology
Apple stem grooving virus ( ASGV ) is one of the economically important latent viruses that are distributed in apple production areas worldwide. The presence of ASGV in apple trees was studied by serological assay and molecular biology methods. A total of 550 apple leaf samples from 14 different areas in Shaanxi were tested by DAS ‐ ELISA , and the results revealed an ASGV infection level of 55%. Those samples were also examined by RT ‐ PCR , and an infection level of 67% was found. Fourteen complete coat protein gene sequences of ASGV were obtained; phylogenetic analysis revealed that these 14 sequences separated into two clusters regardless of the geographic origin or host plants. To our knowledge, this is the first report of molecular variability analysis of ASGV in apple trees in China.