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Detection and Identification of a 16 S r II Group Phytoplasma Causing Clover Little Leaf Disease in I ran
Author(s) -
Hosseini Somayeh,
Bahar Masoud,
Zirak Leila
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12057
Subject(s) - phytoplasma , biology , restriction fragment length polymorphism , broom , botany , polymerase chain reaction , gene , genetics , ecology
White clover plants showing little leaf and leaf reddening symptoms were observed in I sfahan P rovince in central I ran. Restriction fragment length polymorphism analyses of nested PCR ‐amplified fragments from I ranian clover little leaf phytoplasma isolates and representative phytoplasmas from other phytoplasma groups using A lu I , C fo I , K pn I and R sa I restriction enzymes indicated that the clover phytoplasma isolates are related to the peanut WB group. Sequence analyses of partial 16 S r RNA fragments showed that I ranian clover little leaf phytoplasma has 99% similarity with soybean witches'‐broom phytoplasma, a member of the peanut WB (16 S r II ) phytoplasma group. This is the first report of clover infection with a phytoplasma related to the 16 S r II group.