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Identification of Ochratoxigenic A spergillus Section N igri Isolated from Grapes by ITS‐5.8S rDNA Sequencing Analysis and In Silico RFLP
Author(s) -
Lasram Salma,
Oueslati Souheib,
Jouira Hatem Ben,
Chebil Samir,
Mliki Ahmed,
Ghorbel Abdelwahed
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12044
Subject(s) - biology , amplicon , in silico , genbank , internal transcribed spacer , aspergillus niger , aspergillus , ochratoxin a , microbiology and biotechnology , polymerase chain reaction , food science , genetics , mycotoxin , ribosomal rna , gene
To characterize Aspergillus section Nigri strains involved in the ochratoxin A ( OTA ) contamination of Tunisian wine and table grapes, a total of 33 strains were analysed. A molecular characterization of the isolates was performed by the amplification of internal transcribed spacer ( ITS 1‐5.8S rDNA ‐ ITS 2) region combined with amplicon sequencing. Analysis of similarity between the obtained sequences and those deposited in the GenBank database was performed. Twelve strains were confirmed to belong to the Aspergillus carbonarius species. Strains belonging to the Aspergillus niger aggregate group were classified by in silico RFLP assay into two patterns N and T, corresponding to A . niger and A spergillus tubingensis . Among the 21 OTA producing isolates analysed, 13 showed the T ‐type pattern and 8 showed the N ‐type pattern. The presented method showed to be a reliable alternative to the classic RFLP method. Our findings unambiguously revealed that multiple aspergilli species isolated from wine and table grape in Tunisia are able to produce OTA .