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Enzymatic Characterization of C orynespora cassiicola Isolates Using the API ‐ ZYM ® System
Author(s) -
Oliveira Rodrigo Cardoso,
Oliveira Ricardo Ribeiro,
Marchi Bordon Caroline,
Tessmann Dauri José,
SchwanEstrada Kátia Regina Freitas,
Vida João Batista
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12043
Subject(s) - corynespora cassiicola , biology , enzyme , microbiology and biotechnology , host (biology) , botany , biochemistry , genetics , leaf spot
Corynespora cassiicola ( B erk. & C urt.) Wei is an important phytopathogenic fungus, and different isolates show great diversity in their reproductive structures. Therefore, the aim of this study was to evaluate the potential of the API ‐ ZYM ® system as an auxiliary tool in the polyphasic approach of C . cassiicola identification. Five C . cassiicola isolates from different host plants and one P seudocercospora griseola isolate were tested. A typical enzymatic pattern was obtained, with eight enzymes being produced by all five C . cassiicola isolates. An intraspecific differentiation was also found. Certain enzymes produced, such as α‐glucosidase, β‐glucosidase and α‐mannosidase, may be related to pathogenic processes, acting in the degradation of the structural components of the host cells.