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Evaluation of Tomato Hybrids Carrying Ty‐1 and Ty‐2 Loci to Japanese Monopartite Begomovirus Species
Author(s) -
Shahid Muhammad Shafiq,
Ito Takuya,
Kimbara Junji,
Onozato Ayumu,
Natsuaki Keiko T.,
Ikegami Masato
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12041
Subject(s) - begomovirus , biology , leaf curl , geminiviridae , hybrid , virology , tomato yellow leaf curl virus , virus , whitefly , vector (molecular biology) , plant virus , botany , gene , genetics , recombinant dna
Tobacco leaf curl J apan virus, H oneysuckle yellow vein mosaic virus and T omato yellow leaf curl virus are three begomoviruses that infect tomato crops in Japan. Tomato infection by begomoviruses has increased in Japan after the development of a high level of resistance to certain insecticides in some populations of the vector B. tabaci biotypes ‘B and Q’. T y‐1 and T y‐2 homozygous tomato hybrids were evaluated for reaction to monopartite begomovirus species in J apan by A grobacterium ‐mediated inoculation. Test plants were evaluated by a disease assessment scale ( DAS ), varying from 1 = no symptoms to 4 = severe symptoms, and systemic infection was evaluated by polymerase chain reaction ( PCR ), using specific begomovirus primers for each virus. T y‐1 hybrids showed tolerance to HYVMV and with a large number of plants being neither virus‐free nor symptom‐free. The response of T y‐1 hybrids was also resistant to moderately resistant against Tb LCJV . The response of T y‐2 hybrids was resistant to highly resistant against the three monopartite begomoviruses, when compared with susceptible plants.