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Molecular Characterization of Begomoviruses Infecting Sauropus androgynus in Thailand
Author(s) -
Shih SuLing,
Tsai WenShi,
Lee LiMei,
Kenyon Lawrence
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12028
Subject(s) - begomovirus , biology , leaf curl , polymerase chain reaction , virus , virology , geminiviridae , genomic dna , botany , plant virus , veterinary medicine , genetics , dna , gene , medicine
Begomoviruses were detected in leaf samples of S auropus androgynus (L.) Merr. plants showing leaf curling with or without yellowing symptoms in Kamphaeng Saen, Nakhon Pathom, Thailand in 2009 and 2010. From eight plants with symptoms, 17 complete begomoviral DNA ‐As were amplified by polymerase chain reaction and sequenced. No DNA ‐B was detected in any of the plants. All the DNA ‐As had the characteristic begomovirus genome organization of six open reading frames, two in the virion‐sense orientation and four in the complementary orientation. Sequence comparison of these virus isolates indicated that one isolate belongs to T omato leaf curl New Delhi virus, 12 isolates belong to A geratum yellow vein virus and four isolates belong to a novel species with the tentative name Sauropus leaf curl virus. Five of the eight samples were found to be co‐infected by isolates of two different begomovirus species. Recombination analysis indicated that all but one of the isolates were probably the product of one or more recombination events. The results indicated that S . androgynus plants act as natural hosts as well as potential nurseries for genetic recombination between begomovirus species and strains.