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Characterization of Two Fungal Isolates from Cotton and Evaluation of their Potential for Biocontrol of Verticillium Wilt of Cotton
Author(s) -
Zhu HeQin,
Feng ZiLi,
Li ZhiFang,
Shi YongQiang,
Zhao LiHong,
Yang JiaRong
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12027
Subject(s) - verticillium dahliae , biology , verticillium wilt , inoculation , biological pest control , horticulture , bioassay , wilt disease , pathogenicity , malvaceae , botany , veterinary medicine , microbiology and biotechnology , medicine , genetics
Two isolates ( CV d‐ WH w and CV n‐ WH g) recovered from Verticillium‐wilt‐symptomatic cotton grown in H ubei P rovince of C hina were identified based on their morphology, growth characteristics in culture, specific amplification and identification of internal transcribed spacer ( ITS ) r DNA sequence. According to the morphological characteristics, specific PCR amplification and ITS sequences, CV d‐ WH w was identified as V . dahliae and CV n‐ WH g as G ibellulopsis nigrescens . In bioassays, the two isolates had significantly lower pathogenicity to cotton plant than V . dahliae isolate CV d‐ AY b. Cotton pre‐inoculated with isolate CV n‐ WH g or CV d‐ WH w exhibited reduced disease indices of V erticillium wilt compared with those inoculated with CV d‐ AY b alone. Cotton co‐inoculated with CV n‐ WH g or CV d‐ WH w and CV d‐ AY b provided increased protection from subsequent CV d‐ AY b inoculation. These results suggest that the two isolates have the potential to be developed as biocontrol agents for the control of V erticillium wilt in cotton. To our knowledge, this is the first report of a cross‐protection phenomenon using G ibellulopsis nigrescens against V erticillium wilt caused by V . dahliae on cotton.