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Identification of ‘ C andidatus P hytoplasma solani’ Associated with Tree Peony Yellows Disease in C hina
Author(s) -
Gao Ying,
Qiu PingPing,
Liu WenHao,
Su WenMin,
Gai ShuPeng,
Liang YuanCun,
Zhu XiaoPing
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12025
Subject(s) - phytoplasma , biology , primer (cosmetics) , polymerase chain reaction , phloem , virology , candidatus , botany , phylogenetic tree , nested polymerase chain reaction , sieve tube element , 16s ribosomal rna , restriction fragment length polymorphism , genetics , gene , chemistry , organic chemistry
Tree peony ( P aeonia suffruticosais ) plants with yellowing symptoms suggestive of a phytoplasma disease were observed in S handong P eninsula, C hina. Typical phytoplasma bodies were detected in the phloem tissue using transmission electron microscopy. The association of a phytoplasma with the disease was confirmed by polymerase chain reaction ( PCR ) using phytoplasma universal primer pair R 16m F 2/ R 16m R 1 followed by R 16 F 2n/ R 16 R 2 as nested PCR primer pair. The sequence analysis indicated that the phytoplasma associated with tree peony yellows ( TPY ) was an isolate of ‘ C a. P hytoplasma solani’ belonging to the stolbur (16 S r XII ) group. This is the first report of a phytoplasma associated with tree peony.