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Detection and Identification of Two Phytoplasmas (16 S r III ‐ B and 16 S r XII ‐ A ) From Alfalfa ( M edicago sativa ) in S erbia
Author(s) -
Starović Mira,
Kuzmanović Slobodan,
Gavrilović Veljko,
Aleksić Goran,
Popović Tatjana,
Stojanović Saša,
Jošić Dragana
Publication year - 2012
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12010
Subject(s) - phytoplasma , biology , phyllody , restriction fragment length polymorphism , microbiology and biotechnology , polymerase chain reaction , gene , botany , genetics
Plants of alfalfa ( M edicago sativa ) exhibiting general stunting, proliferation and phyllody associated with leaf yellowing and reddening were observed in three localities of C entral S erbia. Phytoplasma strains belonging to 16 S rIII‐ B and 16 S r XII ‐ A groups were detected and identified by RFLP and sequence analysis of 16 S r DNA . Stolbur phytoplasma tuf gene RFLP analysis showed the presence of the T uf AY ‐b‐type phytoplasma subgroup in 80% of symptomatic samples. This is the first report of 16 S r III ‐ B and 16 S r XII ‐ A phytoplasma groups affecting alfalfa in S erbia.