Detection and Identification of Two Phytoplasmas (16 S r III ‐ B  and 16 S r XII ‐ A ) From Alfalfa (  M edicago sativa ) in  S erbia | Zendy

Starović Mira | Zendy; Kuzmanović Slobodan | Zendy; Gavrilović Veljko | Zendy; Aleksić Goran | Zendy; Popović Tatjana | Zendy; Stojanović Saša | Zendy; Jošić Dragana | Zendy

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Detection and Identification of Two Phytoplasmas (16 S r III ‐ B and 16 S r XII ‐ A ) From Alfalfa ( M edicago sativa ) in S erbia

Author(s) -

Starović Mira,

Kuzmanović Slobodan,

Gavrilović Veljko,

Aleksić Goran,

Popović Tatjana,

Stojanović Saša,

Jošić Dragana

Publication year - 2012

Publication title -

journal of phytopathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.53

H-Index - 60

eISSN - 1439-0434

pISSN - 0931-1785

DOI - 10.1111/jph.12010

Subject(s) - phytoplasma , biology , phyllody , restriction fragment length polymorphism , microbiology and biotechnology , polymerase chain reaction , gene , botany , genetics

Plants of alfalfa ( M edicago sativa ) exhibiting general stunting, proliferation and phyllody associated with leaf yellowing and reddening were observed in three localities of C entral S erbia. Phytoplasma strains belonging to 16 S rIII‐ B and 16 S r XII ‐ A groups were detected and identified by RFLP and sequence analysis of 16 S r DNA . Stolbur phytoplasma tuf gene RFLP analysis showed the presence of the T uf AY ‐b‐type phytoplasma subgroup in 80% of symptomatic samples. This is the first report of 16 S r III ‐ B and 16 S r XII ‐ A phytoplasma groups affecting alfalfa in S erbia.

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