Detection and Identification of Group 16 S r VI  Phytoplasma in Willows in China | Zendy

Zhang Lei | Zendy; Li Zhengnan | Zendy; Du Chao | Zendy; Fu Zhaohui | Zendy; Wu Yunfeng | Zendy

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Detection and Identification of Group 16 S r VI Phytoplasma in Willows in China

Author(s) -

Zhang Lei,

Li Zhengnan,

Du Chao,

Fu Zhaohui,

Wu Yunfeng

Publication year - 2012

Publication title -

journal of phytopathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.53

H-Index - 60

eISSN - 1439-0434

pISSN - 0931-1785

DOI - 10.1111/jph.12007

Subject(s) - willow , phytoplasma , biology , phylogenetic tree , primer (cosmetics) , gene , clade , microbiology and biotechnology , genetics , restriction fragment length polymorphism , botany , polymerase chain reaction , chemistry , organic chemistry

In 2010 and 2011, willow proliferation disease was observed in E rdos, I nner M ongolia, C hina. The phytoplasma‐specific 16 S r RNA gene fragment of 1.2 kb was amplified by a nested PCR with universal primer pair P 1/ P 7 followed by R 16 F 2n/ R 2. Phylogenetic and virtual RFLP analyses revealed that the phytoplasma associated with willow proliferation was a member of subgroup 16 S r VI ‐ A . The field survey indicated that the incidence of willow proliferation in Erdos was approximately 36.84%. To our knowledge, this is the first record of group 16 S r VI phytoplasma infecting willow in China.

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