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Molecular Cloning and Characterization of Orthologues of NPR 1 Gene from Poplar
Author(s) -
Shao Yuanhua,
Zhang Hongmei,
He Hongsheng,
Cheng Beijiu,
Xiang Yan
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12002
Subject(s) - wrky protein domain , biology , gene , cloning (programming) , arabidopsis , fusion protein , promoter , microbiology and biotechnology , genetics , molecular cloning , recombinant dna , peptide sequence , gene expression , mutant , computer science , programming language
The non‐expresser of pathogenesis‐related gene 1 ( NPR 1 ) is a significant regulator of systemic acquired resistance in plants. In this study, two homologous poplar genes, P t NPR 1.1 (accession number JQ231218 ), Pt NPR 1.2 (accession number JF732893 ), were identified by bioinformatic analysis and cloned from P opulus deltoids cv . N anlin 95 . A phylogenetic tree was generated from alignments of P t NPR 1 protein sequences and NPR 1‐like genes in other plants. Multiple protein alignments were also constructed to analyse the distribution of crucial domains and highly conserved functional amino acids. Cis‐element analysis revealed that the P t NPR 1 promoters contain RAV 1 AAT and W ‐boxes motifs, both of which are known to be functional cis‐elements of the RAV 1 and WRKY proteins, respectively. The P t NPR 1.1‐ GFP (Green Fluorescent Protein) fusion protein was expressed in Arabidopsis mesophyll protoplasts, where it localized to the cytoplasm. Analysis of transcription levels by RT ‐ PCR revealed expression patterns of P t NPR 1.1 and P t NPR 1.2 in different tissues and following SA and M e JA treatment in different time courses. The results indicated that P t NPR 1.1 and P t NPR 1.2 represent promising candidates for engineering resistance to broad‐spectrum pathogens in poplar.