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Effect of Ultraviolet Radiation on Candida albicans Biofilm on Poly(methylmethacrylate) Resin
Author(s) -
Binns Randold,
Li Wei,
Wu Christine D.,
Campbell Stephen,
Knoernschild Kent,
Yang Bin
Publication year - 2020
Publication title -
journal of prosthodontics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.902
H-Index - 60
eISSN - 1532-849X
pISSN - 1059-941X
DOI - 10.1111/jopr.13180
Subject(s) - candida albicans , sodium perborate , colony forming unit , ultraviolet , corpus albicans , chemistry , significant difference , nuclear chemistry , ultraviolet light , microbiology and biotechnology , materials science , hydrogen peroxide , biology , medicine , biochemistry , bacteria , photochemistry , genetics , optoelectronics
Purpose To investigate the effect of 254‐nm ultraviolet light on the viability of Candida albicans biofilm on poly(methylmethacrylate). Methods Poly(methylmethacrylate) specimens (1 cm × 1 cm × 1 mm) were placed in 6‐well culture plates. Each well contained 8 ml of 10 4 colony forming units/milliliter of C. albicans ATCC90028 and Sabouraud dextrose broth. Plates were incubated at 37 ° C for 24 hours. Specimens were then divided into 11 groups (n = 4): no treatment control groups, 3.8% sodium perborate immersion for 5 minutes (PP5m) and for 12 hours (PO12h), and 6 groups exposed to ultraviolet light for 5, 15, 30, 60, 120 or 300 seconds separately using UVP XX‐15S series lamps. After sonication, cell suspensions were plated, and colony‐forming units were counted. The relationship between survival of C. albicans and ultraviolet light irradiation energy exposure was analyzed and compared to the survival of sodium perborate groups. The effect of disinfection treatments and ultraviolet light energy exposure on C. albicans survival was analyzed with ANOVA (alpha = 0.05). Results There was a significant decrease in C. albicans survival with increasing ultraviolet light energy exposure ( p = 0.00001, p < 0.05) Survival vs. immersion exposure time analysis of chemical disinfection showed no survival of C. albicans in groups PP5m and PO12h. C. albicans in the UV 300s group with energy of 210 mJ/cm 2 (71 CFU/ml) showed a statistical difference from that of two chemical immersion groups (PP5m, PO12h = 0 CFU/ml) ( p = 0.00001, p < 0.05). Conclusion Ultraviolet 254 nm irradiation demonstrated a significant inhibition of C. albicans survival on poly(methylmethacrylate) samples. Ultraviolet light exposure of 300 seconds inhibited the survival of C. albicans close to the level of 3.8% sodium perborate treatment.