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Surface Coating of Gypsum‐Based Molds for Maxillofacial Prosthetic Silicone Elastomeric Material: Evaluating Different Microbial Adhesion
Author(s) -
Khalaf Salah,
Ariffin Zaihan,
Husein Adam,
Reza Fazal
Publication year - 2017
Publication title -
journal of prosthodontics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.902
H-Index - 60
eISSN - 1532-849X
pISSN - 1059-941X
DOI - 10.1111/jopr.12460
Subject(s) - silicone , elastomer , adhesion , materials science , biofilm , scanning electron microscope , composite material , surface modification , coating , silicone elastomers , chemistry , bacteria , genetics , biology
Purpose To compare the adhesion of three microorganisms on modified and unmodified silicone elastomer surfaces with different surface roughnesses and porosities. Materials and Methods Candida albicans , Streptococcus mutans , and Staphylococcus aureus were incubated with modified and unmodified silicone groups (N = 35) for 30 days at 37°C. The counts of viable microorganisms in the accumulating biofilm layer were determined and converted to cfu/cm 2 unit surface area. A scanning electron microscope (SEM) was used to evaluate the microbial adhesion. Statistical analysis was performed using t‐ test, one‐way ANOVA, and post hoc tests as indicated. Results Significant differences in microbial adhesion were observed between modified and unmodified silicone elastomers after the cells were incubated for 30 days ( p < 0.001). SEM showed evident differences in microbial adhesion on modified silicone elastomer compared with unmodified silicone elastomer. Conclusions Surface modification of silicone elastomer yielding a smoother and less porous surface showed lower adhesion of different microorganisms than observed on unmodified surfaces.