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Candida albicans Adherence to Denture Base Material: Chemical Disinfection and the Effect of Acquired Salivary Pellicle Formation
Author(s) -
Rodríguez Acosta Emilio José T.,
Silva Paulo Mauricio Batista,
Jacobina Matheus,
Lara Vanessa Soares,
Neppelenbroek Karin Hermana,
Porto Vinicius Carvalho
Publication year - 2015
Publication title -
journal of prosthodontics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.902
H-Index - 60
eISSN - 1532-849X
pISSN - 1059-941X
DOI - 10.1111/jopr.12197
Subject(s) - candida albicans , saliva , corpus albicans , sodium hypochlorite , microbiology and biotechnology , distilled water , adhesion , acridine orange , acrylic resin , chlorhexidine , chemistry , staining , biology , dentistry , chromatography , medicine , biochemistry , organic chemistry , coating , genetics
Purpose The aim of this study was to evaluate the effect of 1% sodium hypochlorite (H1%) and 4% chlorhexidine gluconate (CG4%) on the adhesion of Candida albicans to denture base acrylic resins, as well as to verify the effect of the acquired salivary pellicle (ASP) formation on this process. Materials and Methods A total of 300 acrylic specimens were immersed in distilled water (control) (n = 100), H1% (n = 100), or CG4% (n = 100) for 30 days. Twenty specimens were used in each experimental period (0, 1, 7, 15, 30 days). At the end of disinfection testing periods, 10 specimens of each group were exposed to human whole saliva to simulate ASP formation, and then all specimens were incubated with C. albicans ATTC 90028. Microorganism adhesion was analyzed by fluorescence microscopy, after staining with Acridine orange. Results In the 30 th disinfection cycle in relation to baseline, the H1% or CG4%, without ASP formation, reduced the C. albicans adhesion by approximately 80%; however, with ASP, this reduction after disinfection with H1% was higher (88%). The presence of ASP resulted in higher reduction of adhered fungal cells in comparison to resin without ASP, at the 1 st H1% or CG4% disinfection cycle, as well as at 30 th H1% disinfection cycles. Conclusions Our results suggest that the presence of saliva might influence the adhesion of C. albicans and improve the effectiveness of methods to reduce fungal adhesion.