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Development of a New Model System to Study Microbial Colonization on Dentures
Author(s) -
Wu Tingxi,
Hu Wei,
Guo Lihong,
Finnegan Marybeth,
Bradshaw David J.,
Webster Paul,
Loewy Zvi G.,
Zhou Xuedong,
Shi Wenyuan,
Lux Renate
Publication year - 2013
Publication title -
journal of prosthodontics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.902
H-Index - 60
eISSN - 1532-849X
pISSN - 1059-941X
DOI - 10.1111/jopr.12002
Subject(s) - dentures , biofilm , colonization , candida albicans , dentistry , stomatitis , microbiology and biotechnology , chemistry , medicine , bacteria , biology , genetics , dermatology
Purpose Dentures are often colonized with a variety of microorganisms, including Candida albicans , that contribute to denture stomatitis. Several in vitro models have been previously established to study denture‐related microbial colonization and evaluate treatment efficacy of denture cleansers; however, those models typically fail to appreciate the complex topology and heterogeneity of denture surfaces and lack effective ways to accurately measure microbial colonization. The purpose of this study was to study microbial colonization with a new model system based on real dentures, to more realistically mimic in vivo conditions. Materials and Methods Scanning electron microscopy was used to observe topological structures among surfaces from different parts of the denture. Employing C. albicans as a model microorganism, we established microbial colonization on different denture surfaces. Moreover, we applied a modified MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyl tetrazolium bromide) colorimetric assay to quantify C. albicans colonization on dentures without the necessity of biofilm removal and to evaluate treatment efficacy of denture cleansers. Results There were significant variations in topological structures among surfaces from different parts of the denture, with the unpolished side having the highest amounts of indentations and pores. The distinct denture surfaces support microbial colonization differently, with the unpolished side containing the highest level of microbial colonization and biofilm formation. Furthermore, the modified MTT colorimetric assay proved to be an accurate assay to measure biofilm formation on dentures and evaluate treatment efficacy of denture cleansers. Conclusion This new denture model system in conjunction with the MTT colorimetric assay is a valuable tool to study denture‐related microbiology and treatment approaches.