Premium
Signaling alterations in oral keratinocytes in response to shisha and crude tobacco extract
Author(s) -
Babu Niraj,
Patil Shankargouda,
Mohan Sonali V.,
Subbannayya Tejaswini,
Advani Jayshree,
Datta Keshava K.,
Rajagopalan Pavithra,
Bhat Firdous Ahmad,
Sidransky David,
Gowda Harsha,
Chatterjee Aditi
Publication year - 2021
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.13154
Subject(s) - carcinogenesis , medicine , cancer , biology , cancer research , oral cancers , immunology , bioinformatics , physiology
Background Tobacco consumption in smoking and non‐smoking forms has been consequential in the rise of oral cancer cases. Among different forms, epidemiological studies from Middle Eastern countries and rural parts of northern India have reported increasing association of oral cancer with waterpipe (hookah) smoking. However, molecular mechanisms and role played by waterpipe smoking in the onset of oral carcinogenesis remains unexplored. Methods In this study, immortalized normal human oral keratinocytes were chronically treated with extracts of two varieties of waterpipe tobacco—crude tobacco and processed shisha. Phenotypic changes and molecular aberrations were examined using cell culture‐based assays and mass spectrometry‐based quantitative proteomic analysis, respectively. Bioinformatics analysis was utilized to analyze proteomics data and identify dysregulated pathways. Results Our data indicate that chronic treatment with waterpipe tobacco extracts increased proliferation, invasion, migration, and significant dysregulation of protein expression in oral keratinocytes. Altered expression of proteins involved in interferon signaling pathway were observed with both varieties of tobacco. Overexpression of cholesterol metabolism and vesicle‐mediated transport proteins were identified exclusively in cells treated with crude tobacco extract. Bioinformatics analyses revealed different oncogenic response in oral cells based on the type of waterpipe tobacco used. Conclusions This study may serve as a useful resource in understanding the early onset of oral cancer attributed to waterpipe smoking.