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Long non‐coding RNA DNM3OS/miR‐204‐5p/HIP1 axis modulates oral cancer cell viability and migration
Author(s) -
Fang Xiaodan,
Tang Zhangui,
Zhang Haixia,
Quan Hongzhi
Publication year - 2020
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.13047
Subject(s) - gene knockdown , microrna , viability assay , cancer research , long non coding rna , biology , cancer cell , rna , cell , cancer , microbiology and biotechnology , cell culture , gene , genetics
Background Non‐coding RNAs play a critical role in the occurrence and development of oral cancer. The present study is aimed to identify long non‐coding RNA (lncRNA) that might be novel effective targets for the treatments of oral cancer and the underlying mechanism. Methods The microarray profiling and RNA‐sequencing analysis were performed to identify lncRNAs related to oral cancer development, and lncRNA DNM3OS was selected. DNM3OS knockdown was generated in cancer cell lines, and the specific effects of DNM3OS knockdown on cell phenotype were examined. DNM3OS targeted miRNA and miRNA targeted downstream mRNA were selected, the predicted bindings were verified, and the specific effects of miRNA on oral cancer cells were examined. Finally, the dynamic effects of DNM3OS and miRNA on target mRNA expression and oral cancer cell phenotype were examined. Results DNM3OS was upregulated in oral cancer tissues and cells. DNM3OS knockdown in CAL27 and SCC‐9 cells inhibited cell viability and migration. DNM3OS targeted miR‐204‐5p to inhibit miR‐204‐5p expression. miR‐204‐5p overexpression suppressed oral cancer cell aggressiveness. miR‐204‐5p targeted HIP1 to inhibit HIP1 expression. HIP1 knockdown inhibited oral cancer cell viability and migration. The effects of DNM3OS knockdown were significantly reversed by miR‐204‐5p inhibition. Within oral carcinoma tissue samples, expression of DNM3OS and HIP1 was increased whereas the miR‐204‐5p expression was downregulated; miR‐204‐5p had a negative correlation with DNM3OS and HIP1, respectively, while DNM3OS and HIP1 were positively correlated with each other. Conclusion Long non‐coding RNA DNM3OS, miR‐204‐5p, and HIP1 form an axis that modulates oral cancer cell viability and migration.