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Evaluation of circulating serum 3 types of microRNA as biomarkers of oral squamous cell carcinoma; A pilot study
Author(s) -
Karimi Abbas,
Bahrami Naghmeh,
Sayedyahossein Amirsalar,
Derakhshan Samira
Publication year - 2020
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12959
Subject(s) - basal cell , microrna , medicine , carcinoma , significant difference , case control study , cell , gastroenterology , oncology , pathology , gene , biology , biochemistry , genetics
The microRNAs are molecules which have important biologic role and play key point in cancers. The aim of present study was to determine the miR‐21, miR‐24, and miR‐29a expression in serum of patients with oral squamous cell carcinoma. Materials and methods Blood samples were obtained from 40 patients (20 in cases and 20 in control group) to determine the miR‐21, miR‐24, and miR‐29a expressions by using real‐time PCR and ΔCT. Results Mean miR‐29a was −2.28 ± 2.15 and 5.61 ± 2.38 in case and control groups, respectively. The miR‐21 was 6.90 ± 3.86 and −0.88 ± 2.31 in case and control groups, respectively. According to the results, miR‐24 was 2.13 ± 2.89 and −0.35 ± 2.44 in case and control, respectively. A significant difference was observed on miR‐21, miR‐24, and miR‐29a between two groups ( P < .05). The results obtained by t test showed miR‐21 and miR‐24 were higher and miR‐29a was lower in plasma of oral squamous cell carcinoma patients and this differences were significant ( P < .05). Conclusion These results suggested miR‐21, miR‐24, and miR‐29a in serum of patients with oral squamous cell carcinoma comparing with normal group can be used as potent markers for carcinoma detection and also may be a potentially therapeutic approach in the future. More longitudinal studies with larger samples are necessary to confirm these findings.