Premium
RALBP1 regulates oral cancer cells via Akt and is a novel target of miR‐148a‐3p and miR‐148b‐3p
Author(s) -
Ieong ChengCheng,
Ma Junpeng,
Lai Wenli
Publication year - 2019
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12936
Subject(s) - carcinogenesis , biology , downregulation and upregulation , cancer research , protein kinase b , microrna , transfection , anaerobic glycolysis , cell , epigenetics , cell culture , phenotype , cancer cell , cancer , microbiology and biotechnology , signal transduction , gene , biochemistry , genetics
Background Malignant tumors arising from the epithelium of the oral cavity are termed as squamous cell carcinomas (OSCC). The aim of the current work was to understand the role of an isoform of RAS‐like protein (RAL), RALBP1, in mediating squamous cell tumorigenesis. The study also aims to understand epigenetic modifications of RALBP1 mediated through microRNA‐148a/b‐3p. Methods Biopsies of tumor and healthy tissues from 25 patients with OSCC were collected and subjected to RNA and protein extraction to confirm upregulation of RLBP1 in tumor tissues. Expression of RLBP1 was silenced in SCC‐9, using shRNA, and HN6 was transfected with plasmid bearing genes for RLBP1 over expression. Tumorigenic traits such as increased glucose uptake, aerobic glycolysis, enhanced cellular survival, cell migration, and invasion were assessed. Probable, molecular machinery involved in the upregulation was also assessed using Western blots. Using Target Scan tool, the miRNAs targeting RLBP1 were identified. Rescue of phenotypes in presence of miRNAs were also evaluated. Results Over expression of RLBP1 was associated with increased glucose uptake and aerobic glycolysis mediated ATP synthesis. The cells divided at a faster rate with a higher rate of migration and invasion phenotype. miR‐148a/b‐3p were found to target RLBP1 and rescued RLBP1 mediated phenotype. Conclusion RLBP1 may mediate squamous cell tumorigenesis in oral cavity, independently of the RAS protein and through Akt. miR‐148a/b‐3p functions as a tumor suppressor by targeting RLBP1.