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Recombinant human interleukin 17A enhances the anti‐ Candida effect of human oral mucosal epithelial cells by inhibiting Candida albicans growth and inducing antimicrobial peptides secretion
Author(s) -
Jiang Lanlan,
Fang Meifei,
Tao Renchuan,
Yong Xiangzhi,
Wu Tiantian
Publication year - 2020
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12889
Subject(s) - candida albicans , corpus albicans , microbiology and biotechnology , biology , beta defensin , recombinant dna , defensin , hypha , antimicrobial , biochemistry , gene
Background Candida albicans ( C albicans ) is the most common fungal pathogen causing opportunistic infections. IL17 (IL17A) is a vital mediator of antifungal immunity. The aim of the study was to investigate the effect of recombinant human interleukin 17A (rhIL17A) on human oral mucosal epithelial cells (hOMECs) defending against C albicans infection. Methods Human oral mucosal epithelial cells were divided into four groups: C albicans + (MOI = 0.1), rhIL17A+ (100 μg/L), rhIL17A + C albicans + (MOI = 0.1, rhIL17A:100 μg/L) and blank control. Then, C albicans growth was observed after 24 hours. Human beta‐2 defensin (hBD‐2), S100A8 and LL‐37 in supernatants and their mRNAs in cells were measured by enzyme‐linked immunosorbent assay and reverse transcription‐polymerase chain reaction, respectively. Results In C albicans + group, C albicans hyphae formation and the death of infected hOMECs were observed. However, in the rhIL17A + C albicans + group, IL17 inhibited both hypha formation, and C albicans from infecting hOMECs and its further growth. There was no statistical significance in adhesion rates of C albicans to hOMECs. Compared with the control group, the level of hBD‐2 mRNA has increased, while hBD‐2 and hBD‐2 mRNA levels in the rhIL17A + C albicans + group were the highest. Both hBD‐2 and hBD‐2 mRNA levels were higher in the rhIL17A+ group than in the C albicans + group. S100A8 and LL‐37 mRNAs have similar trend, and both upregulated after treatment with rhIL17A; however, protein levels were undetectable. Conclusion Recombinant human interleukin 17A may inhibit C albicans from infecting hOMECs by affecting the growth and reproduction of C albicans as well as the formation of hyphae. Besides, rhIL17A might induce hBD‐2, S100A8 and LL‐37 secretion from hOMECs to strengthen their anti‐infective ability.