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Effect of technetium‐99 conjugated with methylene diphosphonate ( 99 Tc‐ MDP ) on OPG / RANKL / RANK system in vitro
Author(s) -
Shen Shiying,
Wang Weihong,
Yang Chun,
Xu Biao,
Zeng Ling,
Qian Yemei
Publication year - 2019
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12801
Subject(s) - rankl , flow cytometry , cell culture , cell growth , apoptosis , in vitro , chemistry , cell , resorption , microbiology and biotechnology , western blot , andrology , endocrinology , medicine , biology , biochemistry , receptor , genetics , activator (genetics) , gene
Background RANKL and RANK play an important role in jaw resorption during the development of the ameloblastomas. Therefore, the aim of this study was to explore the effect of 99 Tc‑ MDP on OPG / RANKL / RANK system on RAW 264.7 and MC 3T3‐E1 cell lines in vitro and provide the theoretical basis for the clinical treatment of the jaw ameloblastoma. Methods Different concentrations of 99 Tc‐ MDP were used to treat RAW 264.7 and MC 3T3‐E1 cell lines. The cell proliferative inhibition rate was analyzed by CCK ‐8. Cell apoptosis and cell cycle were detected by flow cytometry. Western blot was used to detect the expression of OPG , RANKL , and RANK . Results Treatment of RAW 264.7 cell lines with different concentrations of 99 Tc‐ MDP had inhibitory effects and decreased the expression of RANK protein. The cell proliferation of 99 Tc‐ MDP on MC 3T3‐E1 cell lines was stronger at 48 hours than at 24 hours except for 100 μg/mL concentration group. Compared with the concentration of 0.01 μg/mL, the treatment of MC 3T3‐E1 cells with 100 μg/mL 99 Tc‐ MDP showed that the cell proliferative effect decreased at 24 hours and 48 hours ( P < 0.05). After treatment with 0.01 μg/mL 99 Tc‐ MDP , the expression of OPG in MC 3T3‐E1 cells was significantly increased ( P < 0.05). Compared with 0.01 μg/mL, the expression of RANKL was decreased after treatment with 100 μg/mL 99 Tc‐ MDP ( P < 0.05). Conclusion 99 Tc‐ MDP can induce apoptosis of RAW 264.7 cells and inhibit the expression of RANK protein. The effect of 0.01 μg/mL of low concentration of 99 Tc‐ MDP can promote the proliferation of MC 3T3‐E1 cells and increase the expression of OPG and RANKL protein. 99 Tc‐ MDP may have adjuvant therapeutic effects on the treatment of jaw ameloblastoma.