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Role of Noxa in proliferation, apoptosis, and autophagy in human adenoid cystic carcinoma
Author(s) -
Liang Lizhong,
Weng Junquan,
You Yingying,
He Qifen,
Fan Yunping,
Liao Guiqing
Publication year - 2019
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12787
Subject(s) - autophagy , adenoid cystic carcinoma , apoptosis , biology , salivary gland , cancer research , ectopic expression , activator (genetics) , gene , carcinoma , genetics , biochemistry
Background Noxa, which is subset of the Bcl‐2 family of proteins, was previously reported to have considerable therapeutic potential in diverse cancers. However, its expression and role in salivary gland adenoid cystic carcinoma ( ACC ) have not been well studied. This study aimed to elucidate the expression and role of Noxa in salivary gland ACC . Materials and Methods The expression levels of NOXA and its association with overall survival in salivary gland ACC were analyzed by quantitative real‐time PCR . We next examined the effects of Noxa overexpression or inhibition on colony formation, proliferation, apoptosis, and autophagy of salivary gland ACC cells. Furthermore, promoter analysis was performed to identify the potential transcriptional activator of NOXA . Results NOXA was markedly down‐regulated and significantly correlated with a more aggressive phenotype and poor overall survival of salivary gland ACC . Ectopic expression of Noxa suppressed the viability and growth of ACC cells, which involved the induction of apoptosis and autophagy. Moreover, the transcriptional activity of NOXA gene could be enhanced by p53. Conclusion The findings of this study indicate that Noxa, activated transcriptionally by p53, suppress the progression of ACC , whereby it regulates proliferation, apoptosis, and autophagy.