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Leptin impairs the therapeutic effect of ionizing radiation in oral squamous cell carcinoma cells
Author(s) -
da Rocha Rogério Gonçalves,
Santos Eliane Macedo Sobrinho,
Santos Eloá Mangabeira,
Gomes Emisael Stênio Batista,
Ramos Guilherme Veloso,
Aguiar Karina Marini,
Gonçalves Bruno Rodrigues,
Santos Sérgio Henrique Sousa,
De Paula Alfredo Maurício Batista,
Guimarães André Luiz Sena,
Farias Lucyana Conceição
Publication year - 2019
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12786
Subject(s) - leptin , cancer research , carcinogenesis , biology , cell growth , cancer , cancer cell , ionizing radiation , cell , medicine , endocrinology , irradiation , biochemistry , physics , nuclear physics , obesity
Purpose Leptin, an important hormone controlling energy homeostasis, has been linked to the pathogenesis of oral squamous cell carcinoma ( OSCC ). Evidence indicates that head and neck cancer patients undergoing radiotherapy show decreased leptin levels after radiotherapy treatment. Thus, we investigated, through phenotypic and molecular analyses, whether leptin can compromise the therapeutic effect of ionizing radiation and neoplastic behavior of OSCC cells. Methods The human OSCC ‐derived cell lines SCC 9 and SCC 4 were treated with human recombinant leptin and exposed to 6 Gy of irradiation. We performed the in vitro assays of cell migration, death, proliferation, and colony‐forming ability. The reactive oxygen species ( ROS ) levels and proteome analysis by mass spectrometry were also conducted. Results Leptin was able to increase cell proliferation, migration, and colony‐forming ability, despite the suppressive effect induced by irradiation. Furthermore, the leptin promoted a significant reduction of ROS intracellular accumulation, and increased expression of the cancer‐related proteins, as ACTC 1, KRT 6A, and EEF 2 in irradiated OSCC cells. Conclusions Our findings suggest that leptin impairs responsivity of OSCC cells to the ionizing radiation, reducing the suppressive effects of irradiation on the neoplastic phenotype, and increasing protein expression critical to carcinogenesis.