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Prognostic impact of combined immunoprofiles in oropharyngeal squamous cell carcinoma patients with respect to AJCC 8th edition
Author(s) -
Gurin Dominik,
Slavik Marek,
Hermanova Marketa,
Shatokhina Tetiana,
Sana Jiri,
Kazda Tomas,
Selingerova Iveta,
Ahmad Parwez,
Smilek Pavel,
Horakova Zuzana,
Hendrych Michal,
Slampa Pavel,
Slaby Ondrej
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12772
Subject(s) - medicine , oncology , stage (stratigraphy) , immunohistochemistry , hazard ratio , proportional hazards model , radiation therapy , cancer , t stage , biopsy , epidermal growth factor receptor , confidence interval , paleontology , biology
Objectives To examine combined immunoprofiles of epidermal growth factor receptor (EGFR), CD 44, and p16 in oropharyngeal squamous cell carcinoma ( OPSCC ) and to correlate them with radiotherapy treatment outcomes and clinicopathological parameters. Prognostic impact of the American Joint Committee on Cancer ( AJCC ) 8th edition staging system in comparison with 7th edition was analyzed. Methods The study included 77 OPSCC patients treated by definitive intensity‐modulated radiotherapy ( IMRT ). Clinical staging was assessed according to the AJCC , both 7th and 8th edition. Immunohistochemical ( IHC ) analysis of CD 44 and EGFR was performed on primary biopsy tumor tissues. To evaluate the HPV status, IHC detection of p16 was employed. Results The AJCC 8th edition staging system revealed correlations between overall survival ( OS ), progression‐free survival ( PFS ), locoregional control ( LRC ), and clinical stage. EGFR and CD 44 positivity (+) and p16 negativity (−) were associated with clinical stage IV of the disease. CD 44+ and EGFR + OPSCC displayed worse OS and LRC , and these cases also showed the worst 3‐year OS and LRC . Combined analysis of protein expressions identified an association between p16− and EGFR +, p16− and CD 44+, EGFR +, and CD 44+. Combined immunoprofiles CD 44+/p16−, EGFR +/p16−, and EGFR +/ CD 44+ were associated with worst OS and LRC . Conclusions Combined immunoprofiles of p16, EGFR , and CD 44 might provide valuable prognostic and predictive information for the individual OPSCC patients, especially in terms of response to IMRT and prediction of treatment outcomes. Application of the AJCC 8th edition staging for HPV + OPSCC proved to improve hazard discrimination and prognostication of OPSCC .