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Salivary S100A8/A9 in Sjögren's syndrome accompanied by lymphoma
Author(s) -
Jazzar Ahoud A.,
Shirlaw Penelope J.,
Carpenter Guy H.,
Challacombe Stephen J.,
Proctor Gordon B.
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12763
Subject(s) - saliva , medicine , lymphoma , biomarker , gastroenterology , s100a8 , case control study , autoimmune disease , immunology , disease , pathology , inflammation , biology , biochemistry
Background Sjögren's syndrome ( SS ) is an autoimmune inflammatory disease that affects the exocrine glands. The absence of early diagnostic markers contributes to delays in its diagnosis. Identification of changes in the protein profile of saliva is considered one of the promising strategies for the discovery of new biomarkers for SS . Objective To identify salivary protein biomarkers with potential for use in discriminating between different lymphoma risk subgroups of SS . Method Parotid and whole mouth saliva samples were collected from patients with SS , including those in subgroups at higher risk of developing or with confirmed lymphoma, non‐ SS sicca disease controls and healthy subjects. An initial proteomics analysis by mass spectrometry ( LCMSMS ) identified S100A8/A9 as a biomarker and was followed by validation with an enzyme‐linked immunosorbent assay ( ELISA ). Results Significant differences were found in levels of S100A8/A9 in parotid saliva but not whole mouth saliva between patients with SS compared with healthy and disease control subjects ( P = 0.001 and 0.031, respectively). Subgroups of patients with SS based on lymphoma risk showed significant differences in salivary levels of S100A8/A9. Conclusion The results suggest that salivary levels of S100A8/A9 can aid in differentiating between SS , disease control and healthy control subjects, especially the subgroups of SS with lymphoma or at higher risk of lymphoma.