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PAI‐1, CAIX, and VEGFA expressions as prognosis markers in oral squamous cell carcinoma
Author(s) -
Peterle Gabriela Tonini,
Maia Lucas Lima,
Trivilin Leonardo Oliveira,
Oliveira Mayara Mota,
Santos Joaquim Gasparini,
Mendes Suzanny Oliveira,
Stur Elaine,
Agostini Lidiane Pignaton,
Rocha Lília Alves,
Moysés Raquel Ajub,
Cury Patrícia Maluf,
Nunes Fábio Daumas,
Louro Iúri Drumond,
Santos Marcelo,
Silva Adriana Madeira Álvares
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12721
Subject(s) - vascular endothelial growth factor a , immunohistochemistry , pathology , vascular endothelial growth factor , biology , cancer research , plasminogen activator inhibitor 1 , oncology , medicine , plasminogen activator , vegf receptors
Background In oral squamous cell carcinoma ( OSCC ), the HIF ‐1 complex promotes the expression of genes involved in specific mechanisms of cell survival under hypoxic conditions, such as plasminogen activator inhibitor‐1 ( PAI ‐1), carbonic anhydrase 9 ( CAIX ), and vascular endothelial growth factor A ( VEGFA ). The study aimed to investigate the presence and prognostic value of PAI ‐1, CAIX , and VEGFA in OSCC . Materials and Methods Immunohistochemistry was used to analyze the expressions of these proteins in 52 tumoral tissue samples of patients with OSCC , surgically treated and followed by a minimum of 24 months after surgery. The correlations between protein expressions and clinicopathological parameters and prognosis were analyzed. Results Positive PAI ‐1 membrane expression was significantly associated with local disease relapse ( P = .027). Multivariate analysis revealed that the positive PAI ‐1 membrane expression is an independent marker for local disease relapse, with approximately 14‐fold increased risk when compared to negative expression ( OR = 14.49; CI = 1.40‐150.01, P = .025). Strong PAI ‐1 cytoplasmic expression was significantly associated with the less differentiation grade ( P = .027). Strong CAIX membrane expression was significantly associated with local disease‐free survival ( P = .038). Positive CAIX cytoplasmic expression was significantly associated with lymph node affected ( P = .025) and with disease‐specific survival ( P = .022). Multivariate analysis revealed that the positive CAIX cytoplasmic expression is an independent risk factor for disease‐related death, increasing their risk approximately 3‐fold when compared to negative expression ( HR = 2.84; CI = 1.02‐7.87, P = .045). Positive VEGFA cytoplasmic expression was significantly associated with less differentiation grade ( P = .035). Conclusion Our results suggest a potential role for these expressions profiles as tumor prognostic markers in OSCC patients.