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Association of the NOD‐like receptor 3 ( NLRP 3 ) gene variability with recurrent aphthous stomatitis in the Czech population
Author(s) -
Slezakova Simona,
Borilova Linhartova Petra,
Masopustova Lucie,
Bartova Jirina,
Petanova Jitka,
Kuklinek Pavel,
Fassmann Antonin,
Dusek Ladislav,
Izakovicova Holla Lydie
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12694
Subject(s) - recurrent aphthous stomatitis , genotype , single nucleotide polymorphism , haplotype , immunology , medicine , taqman , allele , population , biology , gene , genetics , polymerase chain reaction , stomatitis , environmental health
Background Recurrent aphthous stomatitis ( RAS ) is a multifactorial disease with unclear etiopathogenesis in which disturbance of immunological processes may be involved. The aim of our study was to investigate three single nucleotide polymorphisms ( SNP s) rs3806265, rs4612666, rs10754558 in NOD ‐like receptor 3 ( NLRP 3 ), the gene encoding the component of inflammasome, in patients with RAS and healthy controls in the Czech population. Methods A total of 207 subjects were included in this case‐control study. Sixty‐four patients with RAS and 143 healthy controls were genotyped by a method based on polymerase chain reaction using 5′ nuclease TaqMan ® assays. Detailed anamnestic, clinical, and laboratory data were obtained from all subjects. Results The allele and genotype frequencies of NLRP 3 polymorphisms (rs10754558 and rs3806265) between both groups were similar. However, statistically significant differences in NLRP 3 rs4612666 genotypes between the patients with RAS and controls were found; carriers of the TT genotype had a higher risk of developing RAS than subjects with the CT + CC genotypes ( OR = 14.69, 95% CI = 1.73‐124.72, P = .004, P corr < .05). No associations between NLRP 3 haplotypes and RAS were observed. Conclusions Our study indicates that the NLRP 3 rs4612666 polymorphism may be involved in the development of RAS in the Czech population.