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O ‐GlcNAcylation in oral squamous cell carcinoma
Author(s) -
Kongkaew Tassaporn,
Aung Win Pa Pa,
Supanchart Chayarop,
Makeudom Anupong,
Langsaard Sarawat,
Sastraruji Thanapat,
Chaiyarit Ponlatham,
Krisanaprakornkit Suttichai
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12680
Subject(s) - immunohistochemistry , phosphorylation , protein kinase b , cancer research , basal cell , grading (engineering) , medicine , cancer , antibody , pathology , microbiology and biotechnology , chemistry , biology , immunology , biochemistry , ecology
Background Two post‐translational mechanisms commonly demonstrated in various cancers are protein phosphorylation and glycosylation by O ‐linked β‐ N ‐acetylglucosamine ( O ‐Glc NA c). However, only phosphorylation of the epidermal growth factor receptor ( EGFR )/Akt pathway has been reported in oral squamous cell carcinoma ( OSCC ). Therefore, we aimed to determine both post‐translational modifications in OSCC tissues and in oral cancer cells compared to normal tissues and oral keratinocytes and to find correlations of these modifications with histological grading. Methods Thirty‐two OSCC and ten normal formalin‐fixed and paraffin‐embedded sections were probed with the anti‐ O ‐Glc NA c, anti‐ O ‐Glc NA c transferase ( OGT ), anti‐phosphorylated‐ EGFR tyr1173 , and anti‐phosphorylated‐Akt ser473 antibodies following standard immunohistochemistry. The immunohistochemical ( IHC ) score was determined using the Fromowitz standard. Whole cell lysates of oral cancer cells and normal oral keratinocytes were immunoblotted with the anti‐ O ‐Glc NA c antibody. Results The median IHC scores of O ‐Glc NA c or OGT between OSCC and normal tissues were not different, whereas those of phosphorylated‐ EGFR tyr1173 and phosphorylated‐Akt ser473 were significantly higher in OSCC than normal tissues ( P < .001 and P < .01, respectively). Similarly, expression of O ‐Glc NA cylated proteins in oral cancer cells and normal oral keratinocytes did not differ. In the OSCC group, the median IHC scores of O ‐Glc NA c and OGT were significantly lower than those of phosphorylated‐ EGFR tyr1173 and phosphorylated‐Akt ser473 ( P < .01 and P < .001, respectively). The IHC scores of O ‐Glc NA c or OGT were not determined to correlate with histological grading. Conclusion Unlike other types of cancers, our findings demonstrate that the levels of O ‐Glc NA cylation are not significantly increased in OSCC tissues or in oral cancer cells and are not associated with the histological grading of OSCC .