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Uncovering the potential of CD 44v/ SYNE 1/miR34a axis in salivary fluids of oral cancer patients
Author(s) -
Shah Kavan,
Patel Shanaya,
Modi Bansri,
Shah Franky,
Rawal Rakesh
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12678
Subject(s) - malignancy , saliva , medicine , clinical significance , disease , biomarker , cancer , stage (stratigraphy) , pathology , oncology , gastroenterology , immunology , biology , genetics , paleontology
Objectives Late‐stage diagnosis is one of the major confounders for poor prognosis of patients with oral cancer owing to lack of a biomarker to diagnose this disease at an early stage. Moreover, till date, invasive biopsies are the only option to assess disease occurrence and progression in this malignancy. Thus, this study aims to identify and assess potential salivary markers in OSCC patients in order to open newer avenues in the field of non‐invasive biopsies. Methodology Bioinformatic‐based analysis was performed to identify potential biomarkers that could be assessed in OSCC patients. The expression patterns of CD 44v and its genetic and epigenetic modulators were assessed in saliva of OSCC patients, leukoplakia, and controls using real‐time and methylation‐specific PCR . Statistical analysis was conducted to understand the significance of these markers in terms of their clinical relevance. Results CD 44v/ SYNE 1/miR34a axis was identified using bioinformatic analysis, and the expression profile of these markers was assessed in saliva of OSCC patients. CD 44v6 and CD 44v10 demonstrated a significantly increased expression, whereas SYNE 1 and miR34a depicted a significantly decreased expression in OSCC patients. Statistical analysis suggested a probable role of CD 44v6, SYNE 1, and miR34a in early stages of the malignancy, whereas a strong association was observed between CD 44v6, CD 44v10, and miR34a expression with locoregional aggressiveness and histopathological conditions. Conclusion Collectively, these findings suggested a plausible role of CD 44v/ SYNE 1/miR34a axis as non‐invasive salivary biomarkers to diagnose this disease at an early stage and predict the early onset of metastasis.