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Effect of 17‐allylamino‐17‐demethoxygeldanamycin (17‐ AAG ) on Akt protein expression is more effective in head and neck cancer cell lineages that retain PTEN protein expression
Author(s) -
Pontes Flávia Sirotheau C.,
Pontes Hélder A. R.,
Souza Lucas L.,
Jesus Adriana S.,
Joaquim Andrea M. C.,
Miyahara Ligia A. N.,
Fonseca Felipe P.,
Pinto Junior Décio S.
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12676
Subject(s) - pten , protein kinase b , cancer research , biology , western blot , head and neck squamous cell carcinoma , mdm2 , pi3k/akt/mtor pathway , microbiology and biotechnology , cell culture , signal transduction , cancer , biochemistry , head and neck cancer , gene , genetics
Objectives The aim of this study was to evaluate the expression of Akt, PTEN , Mdm2 and p53 proteins in three different head and neck squamous cell carcinoma ( HNSCC ) cell lines ( HN 6, HN 19 and HN 30), all of them treated with epidermal growth factor ( EGF ) and 17‐allylamino‐17‐demethoxygeldanamycin (17‐ AAG ), an inhibitor of Hsp90 protein. Material and Methods Immunofluorescence and western blot were performed in order to analyze the location and quantification, respectively, of proteins under the action 17‐ AAG and EGF . Results Treatment with EGF resulted in increased levels of Akt, PTEN and p53 in all cell lineages. The expression of Mdm2 was constant in HN 30 and HN 6 lineages, while in HN 19 showed slightly decreased expression. Under the action 17‐ AAG , in HN 6 and HN 19, the expression of PTEN and p53 proteins was suppressed, while Akt and Mdm2 expression was reduced. Finally, in the HN 30 cell lineage were absolute absence of expression of Akt, Mdm2 and p53 and decreased expression of PTEN . Conclusion These data allow us to speculate on the particular utility of 17‐ AAG for HNSCC treatment through the inhibition of Akt protein expression, especially in the cases that retain the expression of PTEN protein.