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Assessment of biologically aggressive, recurrent glandular odontogenic cysts for mastermind‐like 2 ( MAML 2 ) rearrangements: histopathologic and fluorescent in situ hybridization ( FISH ) findings in 11 cases
Author(s) -
Greer Robert O.,
Eskendri Jeffrey,
Freedman Paul,
Ahmadian Moni,
MurakamiWalter Aline,
VarellaGarcia Marileila
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12658
Subject(s) - fluorescence in situ hybridization , pathology , biology , odontogenic cyst , mucoepidermoid carcinoma , cyst , fish <actinopterygii> , histopathology , carcinoma , medicine , gene , genetics , fishery , chromosome
Background Glandular odontogenic cyst ( GOC ) demonstrates a significant predilection toward localized biologic aggressiveness and recurrence. GOC shares certain histopathologic features with intraosseous mucoepidermoid carcinoma ( IMEC ). The current investigation evaluates a group of recurrent, biologically aggressive GOC s to determine whether any cases demonstrated unique histologic features or mastermind‐like2 ( MAML 2 ) rearrangements common to IMEC . Methods Microscopic slides from 11 previously diagnosed GOC s were stained with hematoxylin and eosin and assessed by 2 study participants for 10 classic histopathologic features required to establish a diagnosis of GOC . Cases were evaluated utilizing break‐apart fluorescent in situ hybridization ( FISH ) analysis for the presence of MAML 2 gene rearrangements. Clinical and demographic data on all patients were recorded. Results The mean age for patients included in the study was 55.27 years with a range of 36 to 72 years. The most common presenting symptom was a jaw expansion, and all cysts presented initially as a unilocular or multilocular radiolucency. Cysts displayed a minimum of 6 of 10 histologic parameters necessary for a diagnosis of GOC . One case demonstrated MAML 2 rearrangements by FISH . That case also showed marked ciliation of cyst‐lining epithelial cells and extensive mucous‐secreting goblet cell proliferation. Conclusion Findings in the current study are in concert with previous investigations, and although this study finds only limited molecular evidence to support the premise that recurrent biologically aggressive GOC s are a precursor to IMEC , detection of MAML 2 rearrangements in 1 case suggests that such a theoretic transition, while rare, is possible.