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Cell proliferation is associated with intensity of tumor budding in oral squamous cell carcinoma
Author(s) -
Marangon Junior Helvécio,
Leão Priscila Laiza Rubim,
Melo Victória Vasconcellos Moreira,
Caixeta Ângela Braga,
Souza Paulo Eduardo Alencar,
Aguiar Maria Cássia Ferreira,
Horta Martinho Campolina Rebello
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12653
Subject(s) - tumor budding , budding , cell growth , biology , pathology , proliferation index , cell , cancer , medicine , microbiology and biotechnology , metastasis , genetics , lymph node metastasis
Background Tumor budding is a morphological marker of cancer invasion, defined as the presence of isolated or small clusters of neoplastic cells at the tumor invasive front. This study aimed to evaluate the association between intensity of tumor budding and cell proliferation in oral squamous cell carcinoma (OSCC). Methods Immunohistochemistry was employed in 163 OSCC samples to detect the cell proliferation marker Ki‐67 and multicytokeratin (to identify OSCC cells in tumor budding evaluation). The Mann‐Whitney test was used to evaluate differences in the cell proliferation index between samples with high‐intensity tumor budding and samples with low‐intensity or no tumor budding. In samples with high‐intensity tumor budding, the Wilcoxon test was used to evaluate differences in the cell proliferation index between the budding area and the area outside the budding. The chi‐square test assessed the association between cell proliferation index and intensity of tumor budding. Results The cell proliferation index was higher in samples with high‐intensity tumor budding than in samples with low‐intensity or no tumor budding ( P < .05). Tumors with high‐intensity tumor budding showed a higher cell proliferation index in the budding area than in the area outside the budding ( P < .05). Finally, samples showing high‐intensity tumor budding were associated with high cell proliferation index ( P < .05). Conclusion Cell proliferation is positively associated with intensity of tumor budding in OSCC. Moreover, in tumors showing high‐intensity tumor budding, the budding area is the location of higher cell proliferation. These findings reinforce the hypothesis that tumor budding is associated with the biological behavior of OSCC.