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Micro RNA profiling reveals dysregulated micro RNA s and their target gene regulatory networks in cemento‐ossifying fibroma
Author(s) -
Pereira Thaís dos Santos Fontes,
Brito João Artur Ricieri,
Guimarães André Luiz Sena,
Gomes Carolina Cavaliéri,
Lacerda Júlio Cesar Tanos,
Castro Wagner Henriques,
Coimbra Roney Santos,
Diniz Marina Gonçalves,
Gomez Ricardo Santiago
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12650
Subject(s) - rna , microrna , gene , gene expression profiling , non coding rna , gene expression , small rna , biology , genetics
Background Cemento‐ossifying fibroma ( COF ) is a benign fibro‐osseous neoplasm of uncertain pathogenesis, and its treatment results in morbidity. Micro RNA s (mi RNA ) are small non‐coding RNA s that regulate gene expression and may represent therapeutic targets. The purpose of the study was to generate a comprehensive mi RNA profile of COF compared to normal bone. Additionally, the most relevant pathways and target genes of differentially expressed mi RNA were investigated by in silico analysis. Methods Nine COF and ten normal bone samples were included in the study. mi RNA profiling was carried out by using TaqMan® OpenArray® Human micro RNA panel containing 754 validated human mi RNA s. We identified the most relevant mi RNA s target genes through the leader gene approach, using STRING and Cytoscape software. Pathways enrichment analysis was performed using DIANA ‐mi RP ath. Results Eleven mi RNA s were downregulated (hsa‐miR‐95‐3p, hsa‐miR‐141‐3p, hsa‐miR‐205‐5p, hsa‐miR‐223‐3p, hsa‐miR‐31‐5p, hsa‐miR‐944, hsa‐miR‐200b‐3p, hsa‐miR‐135b‐5p, hsa‐miR‐31‐3p, hsa‐miR‐223‐5p and hsa‐miR‐200c‐3p), and five were upregulated (hsa‐miR‐181a‐5p, hsa‐miR‐181c‐5p, hsa‐miR‐149‐5p, hsa‐miR‐138‐5p and hsa‐miR‐199a‐3p) in COF compared to normal bone. Eighteen common target genes were predicted, and the leader genes approach identified the following genes involved in human COF : EZH 2 , XIAP , MET and TGFBR 1 . According to the biology of bone and COF , the most relevant KEGG pathways revealed by enrichment analysis were proteoglycans in cancer, mi RNA s in cancer, pathways in cancer, p53‐, PI 3K‐Akt‐, FoxO‐ and TGF ‐beta signalling pathways, which were previously found to be differentially regulated in bone neoplasms, odontogenic tumours and osteogenesis. Conclusion mi RNA dysregulation occurs in COF , and EZH 2 , XIAP , MET and TGFBR 1 are potential targets for functional analysis validation.