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Functional analysis of ESM 1 by si RNA knockdown in primary and metastatic head and neck cancer cells
Author(s) -
Bender Onur,
Gunduz Mehmet,
Cigdem Sadik,
Hatipoglu Omer Faruk,
Acar Muradiye,
Kaya Mesut,
Grenman Reidar,
Gunduz Esra,
Ugur Kadriye Serife
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12648
Subject(s) - gene knockdown , immunofluorescence , cell migration , head and neck squamous cell carcinoma , head and neck cancer , rna interference , cancer cell , cancer research , cancer , cell growth , western blot , cell , small hairpin rna , messenger rna , biology , medicine , pathology , microbiology and biotechnology , cell culture , rna , immunology , gene , antibody , genetics
Background Genetic factors play a large role in cancer, and thus, there is a great desire to understand the effects of different genes in cancer and to also develop gene therapy for better treatments. Therefore, the development of alternative diagnosis and therapy modalities is of utmost importance. The aim of our study was to illuminate the role of ESM 1 (endothelial cell‐specific molecule‐1, also known as Endocan) in proliferation and migration of head and neck cancer, thus helping to pave the way for new treatment modalities and predictive biomarkers. Methods ESM 1 expression was shown with immunofluorescence assay using confocal laser scanning microscope in primary and metastatic head and neck cancer cells. ESM 1 expression was knocked down by RNA interference in head and neck cancer cells. Knockdown efficiency was evaluated by quantitative real‐time RT ‐ PCR and Western blot. Cell proliferation and migration assays were performed by xCELL igence real‐time cell analysis system. Results Immunofluorescence assay showed nuclear localization and high expression of ESM 1 in primary and metastatic head and neck cancer cells. ESM 1 mRNA and protein levels were significantly decreased in ESM 1‐knockdown cells compared to control. ESM 1‐knockdown cells showed reduced proliferation and migration activity when compared to control cells. Conclusion These findings suggest that ESM 1 has roles on proliferation and migration of head and neck cancer cells.