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Three‐dimensional reconstruction with serial whole‐mount sections of oral tongue squamous cell carcinoma: A preliminary study
Author(s) -
Wang Yujia,
Chen Sheng,
Ni Yanhong,
Magee Derek,
Pu Yumei,
Zhou Qian,
Wang Zhiyong,
Zhang Lei,
Huang Xiaofeng,
Hu Qingang
Publication year - 2018
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12644
Subject(s) - tongue , basal cell , medicine , mount , epidermoid carcinoma , tongue neoplasm , carcinoma , oral cavity , pathology , dentistry , computer science , operating system
Objectives Margin status and invasion pattern are prognostic factors for oral tongue squamous cell carcinoma ( OTSCC ). Current methods to identify these factors are limited to 2 D observation; it is necessary to explore 3 D reconstruction with whole‐mount sample to improve the accuracy of analysis. This study aimed to study the tissue preparation, section generation, and 3 D reconstruction with whole‐mount OTSCC specimen. Study design Two OTSCC samples were retrieved from Nanjing Stomatological Hospital, Medical School of Nanjing University. One sample was sliced into 3 equal‐sized pieces and subjected to different processing schedules to determine the best method. The second sample was processed accordingly. Serial whole‐mount sections of the second sample were generated, stained with HE /anticytokine antibody in intersection manner, and scanned into digital images. Digital images were aligned and reconstructed into 3D images with Hetero Genius Medical Image Manager 3D Pathology Add‐On [ HGMIM 3D]. Results Successful serial whole‐mount sections of comparable quality to traditional sections were generated. Three‐dimensional images with serial whole‐mount sections were successfully generated. Conclusions Whole‐mount histopathological 3D reconstruction of OTSCC was successfully generated, providing a solid foundation for comprehensive margin and invasion analysis. Although future study and improvement were needed, whole‐mount histopathological 3D reconstruction proved to be a promising method in OTSCC study.