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Studying the association between methylenetetrahydrofolate reductase ( MTHFR ) 677 gene polymorphism, cardiovascular risk and lichen planus
Author(s) -
Rashed Laila,
Abdel Hay Rania,
AlKaffas Marwa,
Ali Shereen,
Kadry Dina,
Abdallah Sara
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12588
Subject(s) - methylenetetrahydrofolate reductase , hyperhomocysteinemia , homocysteine , medicine , genotype , gastroenterology , risk factor , gene polymorphism , allele , endocrinology , biology , gene , genetics
Background There is a reported relation between hyperhomocysteinemia and lichen planus ( LP ). An increase in homocysteine (Hcy) and the risk of cardiovascular disease ( CVD ) in patients with methylenetetrahydrofolate reductase ( MTHFR ) mutation has been described. Objective To detect MTHFR (C677T) gene polymorphism, and to find its association with CVD risk, Hcy and folic acid levels in patients with LP . Methods This hospital‐based case‐control study included 110 patients with LP : 70 with cutaneous LP ( CLP ) and 40 with oral LP ( OLP ). A total of 120 age‐ and sex‐matched healthy subjects were used as controls. Three millilitre venous blood sample was taken for detection of MTHFR gene polymorphism by PCR ‐ RFLP technique and for measurement of the lipid profile. Hcy and folic acid were measured by ELISA . Hypertension was evaluated. Results There were significantly higher prevalence of hypertension with higher Hcy, triglycerides and cholesterol levels and lower folic acid and HDL levels among patients’ groups. Hypertension with higher Hcy and cholesterol levels together with lower folic acid and HDL levels have been found in OLP when compared to CLP . Patients showed a significant higher percentage of the MTHFR 677 TT genotype ( P =.003) and of the MTHFR 677 T allele ( P =.042) compared to controls. Moreover, there was a higher prevalence of MTHFR 677 T allele in patients with CLP . Conclusion MTHFR 677 gene polymorphism may be a risk factor for the development of the LP , and to predispose these patients to higher risk of CVD .