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Role of sialyl 6‐sulfo Lewis X in antitumor immunity against oral squamous cell carcinoma
Author(s) -
Yoshida Hisato,
Hoshino Hitomi,
Imamura Yoshiaki,
Yoshimura Hitoshi,
Sano Kazuo,
Kobayashi Motohiro
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12585
Subject(s) - cytotoxic t cell , immunohistochemistry , biology , pathology , immunity , sialyl lewis x , cancer research , microbiology and biotechnology , immunology , immune system , inflammation , medicine , in vitro , biochemistry , selectin
Background Tumor‐infiltrating lymphocytes ( TIL s) reportedly play a pivotal role in antitumor immunity against oral squamous cell carcinoma ( OSCC ); however, mechanisms governing TIL recruitment to OSCC tissues remain to be clarified. This study was undertaken to assess a potential association between TIL s and high endothelial venule ( HEV )‐like vessels that express sialyl 6‐sulfo Lewis X (LeX). Methods OSCC tissue sections (n=41) were subjected to immunohistochemistry for sialyl 6‐sulfo LeX and CD 34 to allow quantitation of HEV ‐like vessels. Triple immunohistochemistry for sialyl 6‐sulfo LeX and either CD 3 and CD 20 or CD 4 and CD 8 was conducted to determine which lymphocyte subset is more closely associated with HEV ‐like vessels. Results HEV ‐like vessels expressing sialyl 6‐sulfo LeX were detected in 27 of 41 (65.9%) OSCC cases, and these vessels were more frequently found in early disease (T1/T2 stages) compared with advanced (T3/T4) stages. The number of T cells attached to the inner surface of these HEV ‐like vessels was significantly greater than that of B cells, while the number of CD 4 + helper T cells and CD 8 + cytotoxic T cells did not differ significantly. Interestingly, sialyl 6‐sulfo LeX was also expressed on the membrane of a fraction of OSCC cells, and CD 8 + cytotoxic T cells were almost exclusively found attached to these carcinoma cells. Conclusions Sialyl 6‐sulfo LeX is displayed not only on HEV ‐like vessels but also on OSCC cells and may potentially function in antitumor immunity against OSCC .