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Immunophenotype of neutrophils in oral squamous cell carcinoma patients
Author(s) -
Caldeira Patrícia Carlos,
Vieira Érica Leandro Marciano,
Sousa Alexandre Andrade,
Teixeira Antônio Lúcio,
Aguiar Maria Cássia Ferreira
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12575
Subject(s) - immunophenotyping , lipocalin , cd8 , immunology , medicine , lymphocyte , cd16 , cd3 , flow cytometry , biology , pathology , immune system
Background The aim was to explore the immunophenotype of neutrophils and lymphocytes and the inflammatory mediators in patients with oral squamous cell carcinoma, comparing with controls; and to associate with clinicopathological data. Methods Blood was collected from 13 patients and 13 controls. The immunophenotype of neutrophils (CD66b, CD16, CD11a, arginase‐1), T lymphocytes (CD4, CD8) and the intracellular cytokine production (IL‐10, TNF, IFN‐γ) was evaluated by flow cytometry. Plasma concentration of sVCAM‐1, sTNF‐RI, sTNF‐RII, and IL‐1β was measured by ELISA. MPO, Lipocalin‐2/NGAL, sICAM‐1, and p‐selectin were quantified by Luminex assay. The excised tumors were submitted to immunohistochemistry for neutrophils (CD66b) and lymphocytes (CD3, CD4, CD8). Association with clinical data was explored. P values <.05 were considered significant. Results Patients presented higher percentage of neutrophils and lower lymphocytes, resulting a higher neutrophil/lymphocyte ratio than controls. They also presented higher percentage of neutrophils expressing CD66b + , CD66b + Arginase‐1 + , CD66b + IL10 + , CD66b + TNF + , CD66b + Arginase‐1 + IL‐10 + , and lower CD66b + CD16 + CD11a + and CD66b + Arginase‐1 + TNF + . CD66b + neutrophils were detected in all tumors, with a CD66b + /CD3 + ratio of 0.40. Patients showed higher concentration of plasmatic sVCAM‐1 and lower Lipocalin‐2/NGAL. Patients with good outcome presented lower percentage of neutrophils, higher percentage of lymphocytes, and lower NLR than patients who died. Conclusion The amount and immunophenotype of neutrophils and lymphocytes differ between patients and healthy individuals, with a pro‐tumorigenic profile of neutrophils. As these cells also get within tumor microenvironment, they possibly exert systemic and local functions in cancer pathogenesis. The association of neutrophil count with outcome corroborates recent studies and this merits further investigation for applicability as a prognosticator.