Premium
Comparison of effects of zoledronic acid and clodronate on the bone structure: imaginological and histomorphometrical study in vivo
Author(s) -
Martelli Stephanie Joana Roman,
Damian Melissa Feres,
Gomes Ana Paula Neutzling,
Schinestsck André Ribeiro,
Silva Alexandre Emídio Ribeiro,
Vasconcelos Ana Carolina Uchoa
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12546
Subject(s) - zoledronic acid , medicine , hounsfield scale , h&e stain , saline , adobe photoshop , in vivo , urology , cone beam computed tomography , nuclear medicine , dentistry , pathology , radiology , staining , computed tomography , biology , microbiology and biotechnology , software , computer science , programming language
Objectives To compare histologically and imaginologically the bone structure of rats' mandibles treated with bisphosphonates ( BP s) and rats that did not receive BP s. Methods Thirty‐four rat specimens ( Rattus novergicus , Wistar strain) were divided into three groups: (i) 12 rats treated with zoledronic acid; (ii) 12 rats treated with clodronate; and (iii) the control group, containing 10 rats that received saline solution. All individuals were exposed to cone beam computed tomography ( CBCT ). The images were processed and analyzed to obtain the Hounsfield scores, using the software OsiriX 7.0. Sixty‐eight histological slides were obtained from the specimens and stained with hematoxylin and eosin ( HE ). Using the software Adobe Photoshop CS 6, the histological areas containing non‐vital bone were identified and quantified. Results Non‐vital bone presented positive association with the zoledronic acid and clodronate groups. Statistically, no significant difference in bone density was observed among the groups. Conclusions Based on the results, the BP therapy alone was sufficient to induce osteonecrosis. In addition, the CBCT was not a sensible method for detection of the early signs of bone modification in individuals under BP therapy.