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Erythritol/chlorhexidine combination reduces microbial biofilm and prevents its formation on titanium surfaces in vitro
Author(s) -
Drago Lorenzo,
Bortolin Monica,
Taschieri Silvio,
De Vecchi Elena,
Agrappi Serse,
Del Fabbro Massimo,
Francetti Luca,
Mattina Roberto
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12536
Subject(s) - biofilm , chlorhexidine , microbiology and biotechnology , antimicrobial , erythritol , candida albicans , pseudomonas aeruginosa , chemistry , minimum inhibitory concentration , staphylococcus aureus , bacteria , biology , food science , medicine , dentistry , genetics
Background The purpose of this in vitro study was to evaluate the antibiofilm activity of a novel air‐polishing powder consisting of erythritol and chlorhexidine, assessing its ability to reduce previously grown microbial biofilm and to prevent biofilm formation on titanium surfaces. Methods Clinical strains of Staphylococcus aureus , Pseudomonas aeruginosa , Bacteroides fragilis and Candida albicans isolated from peri‐implantitis lesions were used. Biofilm was grown on sandblasted titanium discs and treated with erythritol/chlorhexidine. The antimicrobial activity was evaluated by determining the minimum inhibitory concentration and the minimum microbicidal concentration. The antibiofilm activity was assessed by semiquantitative spectrophotometric assay and by confocal laser scanning microscopy. Results Erythritol/chlorhexidine displayed an inhibitory and a microbicidal activity against all the tested strains. The spectrophotometric analysis showed that the treatment was effective in both reducing the previously developed biofilm and decreasing biofilm formation on titanium surfaces. Confocal laser scanning microscopy analysis showed a significant reduction of the total biofilm volume, with an increase of the percentage of dead cells of all the microorganisms tested. Conclusions Erythritol/chlorhexidine displayed significant antimicrobial and antibiofilm activity against microorganisms isolated from peri‐implantitis lesions. Due to its properties, it might represent a promising approach for the prevention and treatment of peri‐implant diseases associated to microbial biofilm infections.