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Establishment and characterization of a cell population derived from a dentigerous cyst
Author(s) -
MuñizLino Marcos A.,
RodríguezVázquez Mariana,
ChávezMunguía Bibiana,
OrtizGarcía Josué Z.,
GonzálezLópez Lorena,
HernándezHernández Fidel C.,
LicéagaEscalera Carlos,
GarcíaMuñoz Alejandro,
Rodríguez Mario A.
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12528
Subject(s) - dentigerous cyst , pathology , biology , population , western blot , pathogenesis , microbiology and biotechnology , cyst , immunology , medicine , genetics , environmental health , gene
Background Dentigerous cyst (DC) occurs in approximately 20% of jaw cysts, being the second major common odontogenic cyst, after radicular cyst. This oral lesion has the ability to destroy maxillary bones and could be the origin of several odontogenic tumors. However, molecules implicated in its pathogenesis as well as those involved in its neoplastic transformation remain unknown. Here, we established a cell population derived from a DC as an in vitro model for the study of this oral lesion. Methods Cell culture was performed from a DC from a 44‐year‐old male. Cells were cultured at 37°C in DMEM /F12 medium containing 10% fetal bovine serum. Expression of epithelial markers was analyzed by Western blot and immunofluorescence. Ultrastructural characterization was carried out by transmission electron microscopy. Conditioned media were obtained and characterized by zymography and Western blot. Results Cells showed spindle‐shaped morphology, but they express epithelial markers, such as cytokeratins and the odontogenic ameloblast‐associated protein. The ultrastructural analysis showed well‐formed desmosomes present in adhering contiguous cells, confirming the epithelial lineage of this cell population. Cells also contain several vesicles adjacent to plasma membrane, suggesting an active secretion. Indeed, the analysis of the conditioned medium revealed the presence of several secreted proteins, among them the matrix metalloproteinase‐2. Conclusions Our work provides a useful model to identify the molecular mechanisms involved in the pathogenesis of DC.