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Effect of HIV infection in the micronuclei frequency on the oral mucosa
Author(s) -
Lima Celina Faig,
Alves Monica Ghislaine Oliveira,
Furtado Juvencio Jose Duailibe,
Marcucci Marcelo,
Balducci Ivan,
Almeida Janete Dias
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12527
Subject(s) - micronucleus test , micronucleus , oral mucosa , human immunodeficiency virus (hiv) , tongue , immunopathology , mann–whitney u test , viral load , carcinogenesis , statistical significance , immunology , medicine , feulgen stain , biology , gastroenterology , pathology , cancer , toxicity , staining
Background The genotoxic impact of HIV infection on the oral cavity malignancies is unknown. The aim of this study was to evaluate the effect of HIV infection in micronucleus ( MN ) frequency on the oral mucosa of HIV + patients and establish a relationship with early cytogenetic changes in oral carcinogenesis. Methods Thirty HIV + individuals who are under highly active antiretroviral therapy ( HAART ) and 30 non‐ HIV patients were evaluated. Two smears were taken from the lateral border of the tongue and mouth floor and stained by Feulgen. The frequency of MN was examined in 3000 cells per subject under common microscopy. Results MN analysis showed no significant difference between groups by Mann–Whitney U ‐test for total MN s ( P = 0.178). The presence of single MN was greater in control group with statistical significance ( P = 0.009), while in HIV group, multiple MN s were exhibited in higher mean. Conclusions HIV patients under HAART therapy and low viral load values showed higher frequency of multiple MN s, which, although not statistically significant, may be caused by the action of the Vpr gene, an accessory gene of HIV . These results corroborate the theory of HIV infection cytogenetic damage.