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Treatment of chemically induced oral ulcer using adipose‐derived mesenchymal stem cell sheet
Author(s) -
Lee Doh Young,
Kim HeeBok,
Shim In Kyoung,
Kanai Nobuo,
Okano Teruo,
Kwon Seong Keun
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12517
Subject(s) - medicine , mesenchymal stem cell , wound healing , oral mucosa , adipose tissue , buccal administration , transplantation , stem cell , surgery , pathology , gastroenterology , dentistry , biology , genetics
Background This study investigated the effects of mesenchymal stem cell ( MSC ) sheet transplantation on healing of chemically induced oral ulceration in a rabbit animal model. Methods Oral mucosal ulcers were induced by topical application of filter paper soaked with 70% acetic acid to the anterior gingiva and buccal mucosa of 12 New Zealand white rabbits. The animals were randomly assigned to two groups: with (treatment group, n = 6) or without (control group, n = 6) cell sheets applied to ulcers. Gross findings were sequentially evaluated, and histologic examination was performed on day 7. Results Based on gross inspection, ulceration resolved before day 5 in the treatment group; however, in the control group, healing was incomplete on day 7. In the treatment group, the total area of the ulcer decreased significantly from day 2 to day 5 ( P < 0.001) and from day 5 to day 7 ( P = 0.020), whereas the area decreased significantly from day 5 to day 7 in the control group ( P < 0.001). Histologic and immunofluorescence examination revealed full‐thickness mucosa healing and complete basal cell coverage in the treatment group; in contrast, only partial healing was observed on day 7 in the control group. Conclusions Cell sheet technology using MSC can be an alternative treatment for oral ulcerations in that it can decrease healing time without invasive properties.